马铃薯CHI家族基因鉴定及其对外源水杨酸和茉莉酸的响应分析

doi:10.16420/j.issn.0513-353x.2020-0042

摘要/Abstract

摘要：

利用拟南芥几丁质酶（Chitinase,CHI）基因家族蛋白序列在马铃薯基因组内进行BlastP分析,获得马铃薯CHI家族成员,并对其进行基因结构分析、motif预测、进化树构建、顺式作用元件和组织表达分析。共有26个马铃薯CHI基因成员得到鉴定,其所有成员均含有较少的内含子,其中motif 1 ~ motif 6均含有具有催化功能的GH19结构域。一共检测到166个胁迫或激素响应元件。进化树分析显示,CHI分为6个亚家族。马铃薯几丁质酶基因在不同组织中差异表达并且在水杨酸或茉莉酸处理后诱导表达,暗示水杨酸和茉莉酸对几丁质酶家族有不同的调控作用。

关键词: 马铃薯, 几丁质酶, 基因, 水杨酸, 茉莉酸, 表达分析

Abstract:

In this study,the chitinase（CHI）gene family protein sequences of Arabidopsis thaliana were used for BlastP analysis in potato genome to obtain the CHI gene members of potato. Gene structure analysis,motif prediction,evolutionary tree construction,cis-acting elements and tissue expression analysis were also carried out. Our results showed that 26 members of potato CHI genes were identified and all StuChi gene members contained few introns,and motif 1-motif 6 contained GH19 domain with catalytic function. A total of 166 stress or hormone response elements were detected to participate in the regulation of StuChi gene expression. Evolutionary tree analysis showed that CHI were divided into six subfamilies. The quantitative expression of four tissues and the quantitative response to salicylic acid of StuChi genes showed that the tissue expression specificity indicated the differentiation of their functions.

Key words: potato, chitinase, gene, SA, JA, expression analysis

中图分类号:

S532

荐红举, 万梦圆, 付毅, 丁艺, 琚熙三, 尚丽娜, 李辉, 王季春, 胡柏耿, 吕典秋. 马铃薯CHI家族基因鉴定及其对外源水杨酸和茉莉酸的响应分析[J]. 园艺学报, 2021, 48(1): 83-95.

JIAN Hongju, WAN Mengyuan, FU Yi, DING Yi, JU Xisan, SHANG Lina, LI Hui, WANG Jichun, HU Bogeng, LÜ Dianqiu. Genome-wide Identification and Expression Analysis of Chitinase Gene Family in Potato and Its Response to Exogenous Salicylic Acid and Jasmonic Acid[J]. Acta Horticulturae Sinica, 2021, 48(1): 83-95.

图/表 6

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基因名称 Gene	引物序列（5′-3′） Primer sequence
StuEFLa	F:ATTGGAAACGGATATGCTCCA;R:TCCTTACCTGAACGCCTGTCA
StuChi7	F:CGAATACATTGAACAAAACGCG;R:GTATCATTCTTCGTTGGCTTCC
StuChi11	F:CGTCATCTCAAATTCCGTGTTT;R:CAGTAGTGTTTTGGGAGGTTTG
StuChi17	F:CTAAACGACTTCCTGGTTTTGG;R:CATTGAGTCAACATCACCTTGG
StuChi21	F:ACTTCCCATGAAACTACTGGAG;R:TGCTCTCTAAGGAAGCAGTAAC

基因名称 Gene	引物序列（5′-3′） Primer sequence
StuEFLa	F:ATTGGAAACGGATATGCTCCA;R:TCCTTACCTGAACGCCTGTCA
StuChi7	F:CGAATACATTGAACAAAACGCG;R:GTATCATTCTTCGTTGGCTTCC
StuChi11	F:CGTCATCTCAAATTCCGTGTTT;R:CAGTAGTGTTTTGGGAGGTTTG
StuChi17	F:CTAAACGACTTCCTGGTTTTGG;R:CATTGAGTCAACATCACCTTGG
StuChi21	F:ACTTCCCATGAAACTACTGGAG;R:TGCTCTCTAAGGAAGCAGTAAC

基因 Gene	基因编号 Gene ID	位置 Location	氨基酸数 Number of amino acid	分子量/D MW	pI	外显子数Exon No.	亚细胞定位Subcellular location	分类 Subgroup	亚家族Subfamily
StuChi1	PGSC0003DMG400000073	chr01: 72805788-72807273	298	31 990.93	4.5	1	细胞外 Outside	C	GH18
StuChi2	PGSC0003DMG400000074	chr01: 72808827-72810061	298	31 821.89	4.6	1	细胞外 Outside	C	GH18
StuChi3	PGSC0003DMG400000078	chr01: 72833577-72842485	302	32 363.59	5.9	1	细胞外 Outside	C	GH18
StuChi4	PGSC0003DMG400001528	chr02: 38744461-38745679	252	27 535.83	5.9	3	细胞外 Outside	F	GH19
StuChi5	PGSC0003DMG400001529	chr02: 38749197-38750328	254	27 389.43	4.7	3	细胞外 Outside	F	GH19
StuChi6	PGSC0003DMG400003191	chr07: 334428-335534	368	41 516.91	7.1	1	细胞外 Outside	A	GH18
StuChi7	PGSC0003DMG400004593	chr12: 59618049-59621290	327	36 260.51	7.0	3	细胞外 Outside	D	GH19
StuChi8	PGSC0003DMG400006591	chr03: 44670486-44671710	294	32 248.04	9.3	1	质膜Plasma membrane	C	GH18
StuChi9	PGSC0003DMG400008673	chr02: 19537307-19539201	264	28 748.54	8.8	3	细胞外 Outside	F	GH19
基因 Gene	基因编号 Gene ID	位置 Location	氨基酸数 Number of amino acid	分子量/D MW	pI	外显子数Exon No.	亚细胞定位Subcellular location	分类 Subgroup	亚家族Subfamily
StuChi10	PGSC0003DMG400008796	chr00: 25050182-25052792	323	35 031.61	6.5	3	细胞外 Outside	F	GH19
StuChi11	PGSC0003DMG400008797	chr00: 25056603-25057931	280	29 350.85	8.5	4	细胞外 Outside	F	GH19
StuChi12	PGSC0003DMG400011836	chr07: 292990-294141	383	41 627.01	4.8	1	过氧化物酶体 Microbody （Peroxisome）	B	GH18
StuChi13	PGSC0003DMG400011842	chr07: 294672-296216	355	39 811.97	9.2	2	过氧化物酶体 Microbody （Peroxisome）	B	GH18
StuChi14	PGSC0003DMG400011843	chr07: 303868-306084	372	42 305.99	9.3	3	线粒体基质 Mitochondrial matrix space	A	GH18
StuChi15	PGSC0003DMG400016302	chr06: 39116330-39117653	285	31 468.23	5.9	2	细胞外 Outside	E	GH19
StuChi16	PGSC0003DMG400016302	chr06: 39116330-39117653	136	15 182.02	5.4	1	细胞质 Cytoplasm	E	GH19
StuChi17	PGSC0003DMG400021602	chr09: 61360398-61361921	323	35 986.30	7.9	3	细胞外 Outside	D	GH19
StuChi18	PGSC0003DMG400025063	chr04: 61242553-61244259	276	29 982.95	4.6	2	细胞外 Outside	E	GH19
StuChi19	PGSC0003DMG400025063	chr04: 61242553-61244259	136	14 988.53	4.3	1	细胞质 Cytoplasm	E	GH19
StuChi20	PGSC0003DMG400026853	chr00: 21407639-21408792	316	34 091.35	5.2	3	质膜Plasma membrane	F	GH19
StuChi21	PGSC0003DMG400026854	chr00: 21415307-21416600	320	34 117.04	6.2	3	细胞外 Outside	F	GH19
StuChi22	PGSC0003DMG400026855	chr00: 21419777-21420932	332	35 506.90	8.4	3	细胞外 Outside	F	GH19
StuChi23	PGSC0003DMG400033882	chr05: 44304719-44306659	292	30 945.16	7.5	2	细胞外 Outside	C	GH18
StuChi24	PGSC0003DMG400037480	chr07: 347182-349354	435	49 224.80	6.7	4	质膜Plasma membrane	A	GH18
StuChi25	PGSC0003DMG400040317	chr00: 21299520-21300925	279	29 606.22	8.4	2	细胞外 Outside	F	GH19
StuChi26	PGSC0003DMG402001531	chr02: 38760083-38761491	263	28 819.75	9.2	3	细胞外 Outside	F	GH19

基因 Gene	基因编号 Gene ID	位置 Location	氨基酸数 Number of amino acid	分子量/D MW	pI	外显子数Exon No.	亚细胞定位Subcellular location	分类 Subgroup	亚家族Subfamily
StuChi1	PGSC0003DMG400000073	chr01: 72805788-72807273	298	31 990.93	4.5	1	细胞外 Outside	C	GH18
StuChi2	PGSC0003DMG400000074	chr01: 72808827-72810061	298	31 821.89	4.6	1	细胞外 Outside	C	GH18
StuChi3	PGSC0003DMG400000078	chr01: 72833577-72842485	302	32 363.59	5.9	1	细胞外 Outside	C	GH18
StuChi4	PGSC0003DMG400001528	chr02: 38744461-38745679	252	27 535.83	5.9	3	细胞外 Outside	F	GH19
StuChi5	PGSC0003DMG400001529	chr02: 38749197-38750328	254	27 389.43	4.7	3	细胞外 Outside	F	GH19
StuChi6	PGSC0003DMG400003191	chr07: 334428-335534	368	41 516.91	7.1	1	细胞外 Outside	A	GH18
StuChi7	PGSC0003DMG400004593	chr12: 59618049-59621290	327	36 260.51	7.0	3	细胞外 Outside	D	GH19
StuChi8	PGSC0003DMG400006591	chr03: 44670486-44671710	294	32 248.04	9.3	1	质膜Plasma membrane	C	GH18
StuChi9	PGSC0003DMG400008673	chr02: 19537307-19539201	264	28 748.54	8.8	3	细胞外 Outside	F	GH19
基因 Gene	基因编号 Gene ID	位置 Location	氨基酸数 Number of amino acid	分子量/D MW	pI	外显子数Exon No.	亚细胞定位Subcellular location	分类 Subgroup	亚家族Subfamily
StuChi10	PGSC0003DMG400008796	chr00: 25050182-25052792	323	35 031.61	6.5	3	细胞外 Outside	F	GH19
StuChi11	PGSC0003DMG400008797	chr00: 25056603-25057931	280	29 350.85	8.5	4	细胞外 Outside	F	GH19
StuChi12	PGSC0003DMG400011836	chr07: 292990-294141	383	41 627.01	4.8	1	过氧化物酶体 Microbody （Peroxisome）	B	GH18
StuChi13	PGSC0003DMG400011842	chr07: 294672-296216	355	39 811.97	9.2	2	过氧化物酶体 Microbody （Peroxisome）	B	GH18
StuChi14	PGSC0003DMG400011843	chr07: 303868-306084	372	42 305.99	9.3	3	线粒体基质 Mitochondrial matrix space	A	GH18
StuChi15	PGSC0003DMG400016302	chr06: 39116330-39117653	285	31 468.23	5.9	2	细胞外 Outside	E	GH19
StuChi16	PGSC0003DMG400016302	chr06: 39116330-39117653	136	15 182.02	5.4	1	细胞质 Cytoplasm	E	GH19
StuChi17	PGSC0003DMG400021602	chr09: 61360398-61361921	323	35 986.30	7.9	3	细胞外 Outside	D	GH19
StuChi18	PGSC0003DMG400025063	chr04: 61242553-61244259	276	29 982.95	4.6	2	细胞外 Outside	E	GH19
StuChi19	PGSC0003DMG400025063	chr04: 61242553-61244259	136	14 988.53	4.3	1	细胞质 Cytoplasm	E	GH19
StuChi20	PGSC0003DMG400026853	chr00: 21407639-21408792	316	34 091.35	5.2	3	质膜Plasma membrane	F	GH19
StuChi21	PGSC0003DMG400026854	chr00: 21415307-21416600	320	34 117.04	6.2	3	细胞外 Outside	F	GH19
StuChi22	PGSC0003DMG400026855	chr00: 21419777-21420932	332	35 506.90	8.4	3	细胞外 Outside	F	GH19
StuChi23	PGSC0003DMG400033882	chr05: 44304719-44306659	292	30 945.16	7.5	2	细胞外 Outside	C	GH18
StuChi24	PGSC0003DMG400037480	chr07: 347182-349354	435	49 224.80	6.7	4	质膜Plasma membrane	A	GH18
StuChi25	PGSC0003DMG400040317	chr00: 21299520-21300925	279	29 606.22	8.4	2	细胞外 Outside	F	GH19
StuChi26	PGSC0003DMG402001531	chr02: 38760083-38761491	263	28 819.75	9.2	3	细胞外 Outside	F	GH19