关键词: 辣椒, GRF基因家族, 表达分析, GA3

Abstract: The Growth-Regulating Factors（GRFs）are plant-specific transcription factors that play pivotal roles in growth and development，stress and phytohormone response. However，the GRF family in Capsicum remains to be investigated. In this study，genome-wide retrievation of GRFs in five Capsicum reference genomes was carried out using BLASTP and HMM（Hidden Markov Model），and a total of 52 GRFs were identified. Further analysis was performed regarding their characterization，phylogenetic relationship，tissues expression profiles and expression level upon GA3 treatment. The length of pepper GRF proteins ranges from 200 to 745 amino acids（aa），with the molecular weight（MW）of 23–81 kD，and the theoretical isoelectric point of 5.9–9.5. The majority of GRF proteins harbor the conservative QLQ and WRC domains at N-terminal end，and one or more FFD，TQL，or GGPL motifs in the C-terminus. A few GRFs contain only the WRC domain in the N-terminus and the non-conserved

Key words: Capsicum, GRF gene family, expression analysis, GA3