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园艺学报 ›› 2020, Vol. 47 ›› Issue (10): 2009-2018.doi: 10.16420/j.issn.0513-353x.2019-0942

• 研究报告 • 上一篇    下一篇

福建马铃薯黑胫病病原分离及鉴定

李华伟,林志坚,罗文彬,许国春,纪荣昌,邱思鑫,汤 浩*   

  1. 福建省农业科学院作物研究所,农业农村部南方薯类科学观测实验站,福建省特色旱作物品种选育工程技术研究中心,福州 350013
  • 出版日期:2020-10-25 发布日期:2020-11-10
  • 基金资助:
    福建省公益类科研专项(2018R1026-9);国家现代农业产业技术体系建设专项资金项目(CARS-10-ES11)

Isolation and Identification of the Pathogen Caused Potato Blackleg Disease in Fujian Province

LI Huawei,LIN Zhijian,LUO Wenbin,XU Guochun,JI Rongchang,QIU Sixin,and TANG Hao*   

  1. Crop Research Institute,Fujian Academy of Agricultural Sciences,Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,Technical Research Center of Specialty Day Crop Variety Breeding of Fujian,Fuzhou 350013,China
  • Online:2020-10-25 Published:2020-11-10

摘要: 为明确福建马铃薯黑胫病的病原菌的种类和生物学特性,采集福建3个马铃薯种植区的病样,并通过平板分离、纯化和致病性测定、生理生化测定及分子生物学方法对该病原菌进行鉴定。结果从病样中分离获得9个菌株,其中2个菌株鉴定为黑腐果胶杆菌(Pectobacterium atrosepticum),3个菌株鉴定为胡萝卜软腐果胶杆菌胡萝卜亚种(P. carotovorum subsp. carotovorum),4个菌株鉴定为胡萝卜软腐果胶杆菌巴西亚种(P. carotovorum subsp. brasiliensis)。不同地区菌株差异较大,其中福州青口的病原是P. atrosepticum,霞浦的病原是P. carotovorum subsp. carotovorum,而周宁的是P. carotovorum subsp. carotovorum和P. carotovorum subsp. brasiliense混合侵染。

关键词: 马铃薯, 黑胫病, 病原, 鉴定

Abstract: In order to clarify the species and biological characteristics of the pathogen which caused potato blackleg disease in Fujian Province,the pathogens were collected and isolated from Qingkou,Xiapu and Zhouning,and the pathogens were identified with plate isolation,purification,pathogenicity,physiological and biochemical characteristics and molecular biological methods. The results showed that nine strains of potato blackleg pathogens were isolated from the pathogenic potato,two were identified as Pectobacterium atrosepticum,three as P. carotovorum subsp. carotovorum,and four as P. carotovorum subsp. brasiliensis. And the nine strains were different,the pathogen in Qingkou was P. atrosepticum,that in Xiapu was P. carotovorum subsp. carotovorum,and that in Zhouning was a mixture of P. carotovorum subsp. carotovorum and P. carotovorum subsp. brasiliense.

Key words: potato, blackleg disease, pathogen, identification

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