极早熟油桃幼胚离体培养成苗相关因素探讨

园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 739-742.

极早熟油桃幼胚离体培养成苗相关因素探讨

董晓颖¹ ; 孟新法²; 李培环^1*; 王永章¹; 刘成连¹

(¹ 莱阳农学院园艺学院, 山东青岛266109; ² 中国农业大学农业与生物技术学院, 北京100094)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

Studies on the Correlative Factors for Normal Seedling Development inImmature Embryo Culture of Ultra-early Maturing Nectarine

DONG Xiao-ying¹ ; MENG Xin-fa²; LI Pei-huan^1*; WANG Yong-zhang¹ ; LIU Cheng-lian¹

(¹ College of Horticulture, Laiyang Agricultural University, Qingdao, Shandong 266109, China; ²College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要/Abstract

摘要： 以极早熟油桃‘超红短枝’的幼胚为试材, 在离体培养条件下, 研究了不同胚龄、低温处理
时间、蔗糖浓度、6-BA、GA₃浓度配比及种皮等因素对幼胚发育和成苗的影响。结果表明: 果实发育56 d
时, 平均PF值仅为0.086。在各处理中, 以低温处理80 d、糖浓度6%对幼胚的发育和成苗效果最佳。
6-BA、GA₃配比对经80 d低温处理的幼胚影响较小, 9 mg/L的6-BA对30 d低温处理的幼胚发育有一定的
促进作用。在低温条件下, 带种皮培养对幼胚PF值增大和提高成苗率的效果明显好于不带种皮培养的。

关键词: 油桃, 幼胚培养, PF值, 成苗率

Abstract: The effects of different embryo developmental stages, treatment time of low temperature, 6-BA
and GA₃ levels, sucrose concentration in culture medium and seed coats on immature embryo development and
normal seedling rate were studied with ultra-earlymaturing nectarine variety Super Red Spur. The results indi
cated that the average PF value was only 0.086 when fruits developed up to 56 days. Among the all treat
ments, the treatmentwith low temperature for 80 days and 6% sucrose concentrations in the culture medium
had the highest effects on immature embryo growth and normal seedlings rate. Moreover, different 6-BA and
GA₃ concentrations showed little influence on the immature embryos cultured for 80 days in low temperature;
but 9 mg/L 62BA had some promotion on the immature embryos development for 30 days. The PF value and
the percent of normal seedling of immature embryos with seed coatswere found better compared with the em
bryo without seed coatswhen cultured at low temperature condition.

Key words: Nectarine, Immature embryo culture, PF value, Normal seedling rate

董晓颖;孟新法;李培环;王永章;刘成连. 极早熟油桃幼胚离体培养成苗相关因素探讨[J]. 园艺学报, 2007, 34(3): 739-742.

DONG Xiao-ying;MENG Xin-fa;LI Pei-huan;WANG Yong-zhang;LIU Cheng-lian. Studies on the Correlative Factors for Normal Seedling Development inImmature Embryo Culture of Ultra-early Maturing Nectarine[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 739-742.

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