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园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 104-106.

• 研究报告 • 上一篇    下一篇

低温胁迫下弓葵幼苗膜脂过氧化及保护酶活性的变化

杨盛昌1;谢潮添1;张平1;陈德海1;丁印龙2;廖启2   

  1. (1 厦门大学生命科学学院, 厦门361005 ;2 厦门园林植物园, 厦门361003)
  • 收稿日期:2002-04-03 修回日期:2002-06-05 出版日期:2003-02-25 发布日期:2003-02-25

Changes in Membrane Lipid Peroxidation and Activities of Cell Defense Enzymein Leaves of Butia capitata Becc Seedling under Low Temperature Stress

Yang Shengchang1;Xie Chaotian1;Zhang Ping1;Chen Dehai1;Ding Yinlong2; Liao Qiliao2   

  1. (1Life Science College , Xiamen University , Xiamen 361005 , China ;2 Xiamen Botanical Garden , Xiamen 361003 , China)
  • Received:2002-04-03 Revised:2002-06-05 Online:2003-02-25 Published:2003-02-25

摘要: 低温胁迫下弓葵( Butia capitata Becc) 幼苗叶片的MDA 含量逐渐增加, 膜脂过氧化作用增强。- 8 ℃条件下的膜脂过氧化作用明显强于2 ℃。细胞膜透性在2 ℃条件下变化不大, - 8 ℃时则随低温胁迫时间延长而急剧上升, 细胞膜受到伤害。- 8 ℃胁迫下细胞保护酶SOD、POD 和CAT 活性短期(6 h) 内升高,然后下降, 24 h 以后3 种保护酶受到低温胁迫的严重抑制。在2 ℃胁迫下, SOD 活性在6 h 内变化不大, 随后下降; CAT活性变化趋势与- 8 ℃时相似, 但变化幅度较小; POD 虽也呈现先升后降的趋势, 但降幅明显小于升幅, 至48 h 时POD 活性仍维持较高水平。2 ℃低温胁迫不是抑制而是促进POD 活性的提高。

关键词: 弓葵, 低温胁迫, 膜脂过氧化, 保护酶

Abstract: Under low temperature stress , as a product of membrane liquid peroxidation , malondialdehyde (MDA) content in leaves of Butia capitata seedling gradually increased with elongation of stress time and stress intensity. Membrane lipid peroxidation raised more quickly under - 8 ℃than that under 2 ℃. As for membrane permeability , there was only a little change under 2 ℃, but it showed a sharp increase under - 8 ℃, indicating that cell membrane suffered severe damage after 24 h of - 8 ℃. Cell defense enzymes SOD , POD and CAT activity raised immediately in 6 h and then decreased under - 8 ℃. They were severely inhibited by low temperature stress. Under 2 ℃, SOD activity had little change in 6 h , and then decreased. CAT activity had a similar trend with that under - 8 ℃, but it changed by a lower ranger. Though POD activity also took a trend of increasing firstly and then decreasing , POD activity maintained a high level until 48 h , it gave us a hint that 2 ℃ low temperature stress probably could not inhibit , but promote POD activity in 48 h for leaves of Butia capitata
seedling.

Key words: Butia capitata Becc seedling, Low temperature stress, Membrane lipid peroxidation, Cell defense enzyme

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