春石斛的多倍体诱导

园艺学报 ›› 2009, Vol. 36 ›› Issue (9): 1381-1384.

春石斛的多倍体诱导

郑宝强¹;张莹^1,2;王雁^1*;李振坚¹;朱向涛¹; 律春燕¹

(¹ 中国林业科学研究院林业研究所, 国家林业局林木培育重点试验室, 北京100091; ² 中国林业科学院亚热带林业研究所, 浙江富阳311400)

收稿日期:2008-12-11 修回日期:2009-06-15 出版日期:2009-09-25 发布日期:2009-09-25
通讯作者: 王雁

Polyploid Induction of Nobile-type Dendrobium

ZHENG Bao-qiang¹;ZHANG Ying^1,2;WANG Yan^1*;LI Zhen-jian¹;ZHU Xiang-tao¹;Lü Chun-yan¹

(¹ Research Institute of Forestry, Chinese Academ y of Forestry,Key laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091,China; ²Research Institute of Subtropical Forestry, Chinese Academ y of Forestry, Fuyang, Zhejiang 311400, China)

Received:2008-12-11 Revised:2009-06-15 Online:2009-09-25 Published:2009-09-25
Contact: WANG Yan

摘要/Abstract

摘要： 在离体培养条件下比较了秋水仙素不同浓度、不同处理时间诱导杂种春石斛体细胞染色体加
倍的效果。结果表明, 0.06%秋水仙素处理12 h的诱变效果最佳, 诱变率达69.1%。用组织培养结合不定芽诱导技术获得了稳定的多倍体变异株。变异株与正常株形态差异明显, 气孔数及大小之间差异显著。二倍体对照2n = 2x = 38, 变异株2n = 4x = 76 (四倍体) , 同时也发现存在嵌合体现象。

关键词: 石斛, 春石斛, 多倍体, 秋水仙素, 染色体

Abstract: A hybrid of nobile-type Dendrobium was treated with colchicines at different concentration and time duration in vitro. The results showed that the best effectwas obtained by the treatment of 0.06% colchicines with 12 hours and its induction rate was 69.1%. Comparing the characteristics of plants and stomas,there were remarkable difference between the treatment and the control. Chromosome observation found that the chromosome number of treated plants was 76 and the chromosome number of the control was 38, indicating the tetraploid (2n = 4x = 76) of nobile-type Dendrobium was induced successfully. The mixer-fold phenomenon was also found among treatment plants.

Key words: Dendrobium, nobile-type Dendrobium, polyploid, colchicine, chromosome

中图分类号:

S 682.31

郑宝强;张莹;王雁;李振坚;朱向涛;律春燕. 春石斛的多倍体诱导[J]. 园艺学报, 2009, 36(9): 1381-1384.

ZHENG Bao-qiang;ZHANG Ying;WANG Yan;LI Zhen-jian;ZHU Xiang-tao;Lü Chun-yan. Polyploid Induction of Nobile-type Dendrobium[J]. ACTA HORTICULTURAE SINICA, 2009, 36(9): 1381-1384.

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