文心兰类原球茎液体增殖过程中秋水仙素化学诱变

园艺学报 ›› 2009, Vol. 36 ›› Issue (9): 1385-1389.

文心兰类原球茎液体增殖过程中秋水仙素化学诱变

崔广荣^1,2;上官凌飞¹;张子学²;胡能兵²;张从宇²;侯喜林^1*

(¹南京农业大学作物遗传与种质创新国家重点实验室, 南京210095; ²安徽科技学院植物科学学院, 安徽凤阳233100)

收稿日期:2009-04-30 修回日期:2009-06-25 出版日期:2009-09-25 发布日期:2009-09-25
通讯作者: 侯喜林

Colchicines Chemical Induction During Propagaion of Protocorm-like Bodiesof Oncidium in Liquid Culture

CUI Guang-rong^1,2;SHANGGUAN Ling-fei¹;ZHANG Zi-xue²;HU Neng-bing²; ZHANG Cong-yu²;HOU Xi-lin^1*

(¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University,Nanjing 210095, China; ² Plant Science School of Anhui Science and Technology University,Fengyang, Anhui 233100, China)

Received:2009-04-30 Revised:2009-06-25 Online:2009-09-25 Published:2009-09-25
Contact: HOU Xi-lin

摘要/Abstract

摘要： 采用秋水仙素对液体增殖培养中的文心兰类原球茎进行多倍体诱变, 获得了大量的文心兰多
倍体试管苗。结果表明, 秋水仙素浓度越高, 处理时间越长, 类原球茎受伤害程度越严重, 再生苗多倍体比例越大, 最高诱变率可达46.7%; 低浓度, 短时间秋水仙素处理易产生嵌合体; 四倍体试管苗植株粗短, 健壮, 叶片宽厚, 叶片下表皮气孔张度较大, 下表皮细胞核较大且靠近细胞壁边缘; 根尖细胞染色体数加倍。

关键词: 文心兰, 类原球茎, 多倍体, 秋水仙素, 液体培养, 化学诱变

Abstract:

Colchicines were employed to induce polyploid plantlets of Oncidium during ropagation of protocorm-like bodies ( PLB s) in liquid culture. A lot of polyploid plantlets were obtained by this technique.The results showed that the PLBs cultured in the medium contained higher concentration of colchicines got
harmed more severely and the rate of ployploid plantlets was bigger with higher concentration of colchicines or longer time of treatment . The biggest rate of polyploid plantlets regenerated from the PLBs could reach to 46.7%. The mixoploid could be produced under the condition of lower concentrations of colchicines and short time of treatment. The tetraloid plantlets were shorter and stronger and leaves changed broad and thick. The diameter of lower epidermal stomata in the tetraploid was a little bigger than the one in the diploid. The cell nucleus of leaf lower epidermal became bigger and approached to cell wall. The number of chromosomes in root tip cells of the polyploid plantlets were doubled.

Key words: Oncidium, protocorm-like body, polyploid, colchicine, liquid culture, chemical induction

中图分类号:

崔广荣;上官凌飞;张子学;胡能兵;张从宇;侯喜林. 文心兰类原球茎液体增殖过程中秋水仙素化学诱变[J]. 园艺学报, 2009, 36(9): 1385-1389.

CUI Guang-rong;SHANGGUAN Ling-fei;ZHANG Zi-xue;HU Neng-bing;ZHANG Cong-yu;HOU Xi-lin. Colchicines Chemical Induction During Propagaion of Protocorm-like Bodiesof Oncidium in Liquid Culture[J]. ACTA HORTICULTURAE SINICA, 2009, 36(9): 1385-1389.

[1]	朱炜, 曹津津, 陈曦, 张葳, 孙榕泽, 朱绍才, 赵家庚, 崔雅琦, 王宇暄, 于晓南. 芍药新品种‘粉罗裙’‘千重粉黛’‘笑春风’和‘碧叶粉荷’[J]. 园艺学报, 2023, 50(2): 457-458.
[2]	蒋靖东, 韦壮敏, 王楠, 朱晨桥, 叶俊丽, 谢宗周, 邓秀新, 柴利军. 山金柑四倍体资源的发掘与鉴定[J]. 园艺学报, 2023, 50(1): 27-35.
[3]	方能炎, 樊荣辉, 罗远华, 孔兰, 林榕燕, 叶秀仙, 林兵, 钟淮钦, 黄敏玲. 文心兰OnGI在拟南芥中异源表达促进开花[J]. 园艺学报, 2022, 49(4): 841-850.
[4]	吴婷, 贾瑞冬, 杨树华, 赵鑫, 于晓南, 国圆, 葛红. 蝴蝶兰多倍体育种研究进展与展望[J]. 园艺学报, 2022, 49(2): 448-462.
[5]	李欣, 刘亮, 余让才, 李昕悦, 玉云祎, 岳跃冲, 范燕萍. 文心兰花药帽脱落与切花花朵衰老的关系研究[J]. 园艺学报, 2021, 48(6): 1163-1172.
[6]	叶蓓蕾, 张叶, 刘乐, 黎维诗, 郝代成, 凌鹏, . 文心兰新品种‘海文吉祥1号’[J]. 园艺学报, 2020, 47(S2): 3057-3058.
[7]	张叶, 叶蓓蕾, 刘乐, 黎维诗, 郝代成, 凌鹏, . 文心兰新品种‘海博吉祥1号’[J]. 园艺学报, 2020, 47(S2): 3059-3060.
[8]	郭向阳1，高洁1，宋希强1，凌鹏1，周扬1，郝代成2，黎维诗2，丁琼1,*. 海南岛文心兰病叶组织真菌的分离鉴定与优势真菌致病性初探[J]. 园艺学报, 2020, 47(7): 1369-1382.
[9]	周锐, 解凯东, 王伟, 彭珺, 谢善鹏, 胡益波, 伍小萌, 郭文武, . 依据多倍体形态特征快速高效发掘柑橘四倍体[J]. 园艺学报, 2020, 47(12): 2451-2458.
[10]	党江波1，梁国鲁1，李彩2，吴頔1，郭启高1,*，梁森林1，王鹏1. 果树多倍体砧木的研究现状与展望[J]. 园艺学报, 2019, 46(9): 1701-1710.
[11]	王春夏，尹玥，王志平，严瑞，付麟岚，孙红梅*. 多倍化兰州百合和细叶百合组培苗再生和耐非生物胁迫能力[J]. 园艺学报, 2019, 46(12): 2359-2368.
[12]	孙红梅1,2,*，付麟岚1，王志平1，盖美竹1，王春夏1,2. 基于体细胞胚发生的细叶百合和兰州百合多倍体诱导及鉴定[J]. 园艺学报, 2018, 45(6): 1136-1146.
[13]	李蓉1，吴晓佩1，王雪晶1，陈裕坤1，郭容芳1，林玉玲1，赖钟雄1,，徐涵1,2,. 文心兰RFNR的克隆、亚细胞定位及其与LFNR不同的胁迫响应机制研究[J]. 园艺学报, 2018, 45(11): 2164-2176.
[14]	吕晔，胡兰，王利虎，刘平，刘孟军. 秋水仙素处理后‘无核丰’枣的减数分裂行为及2n花粉的形成[J]. 园艺学报, 2017, 44(1): 11-22.
[15]	刘平1，刘孟军1,*，石庆华1，王玖瑞2，代丽1，胡兰1，王利虎1. 四倍体鲜食酸枣新品种‘珠光’[J]. 园艺学报, 2017, 44(1): 195-196.