马铃薯抗坏血酸含量及其代谢相关酶活性关系的研究

园艺学报 ›› 2009, Vol. 36 ›› Issue (9): 1370-1374.

马铃薯抗坏血酸含量及其代谢相关酶活性关系的研究

秦爱国1;于贤昌2*

(¹山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东泰安271018; ²中国农业科学院蔬菜花卉研究所, 北京100081)

收稿日期:2009-03-31 修回日期:2009-07-13 出版日期:2009-09-25 发布日期:2009-09-25
通讯作者: 于贤昌

Relationship Between Ascorbic Acid Content and the Enzyme ActivitiesInvolved in AsA Metabolism of Potato

QIN Ai-guo¹;YU Xian-chang^2*

(1 State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University,Tai'an, Shandong 271018, China; ²Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing100081, China)

Received:2009-03-31 Revised:2009-07-13 Online:2009-09-25 Published:2009-09-25
Contact: YU Xian-chang

摘要/Abstract

摘要： 为探讨马铃薯不同器官中抗坏血酸(AsA) 含量及其代谢相关酶活性关系, 研究了马铃薯幼
叶、功能叶、老叶、茎和块茎中AsA和其氧化态脱氢抗坏血酸(DHA) 的含量与L - 半乳糖- 1, 4 - 内酯脱氢酶( GalLDH) 、脱氢抗坏血酸还原酶(DHAR) 、谷胱甘肽还原酶( GR ) 、抗坏血酸过氧化物酶(APX) 、抗坏血酸氧化酶(AO) 和单脱氢抗坏血酸还原酶(MDHAR) 等6种酶活性之间的相关性。结果表明, 马铃薯AsA在幼叶和块茎中含量很高。叶片和茎的抗坏血酸库(AsA与DHA之和) 水平与GalLDH活性显著相关, 而AsA含量与DHAR活性显著相关, DHA含量与APX活性显著相关。说明在马铃薯幼叶中高含量的AsA可能由于GalLDH和DHAR的高活性; 而块茎中AsA的积累, 主要来自于叶片的运输和DHAR催化的DHA再生。

关键词: 马铃薯, 抗坏血酸, L - 半乳糖- 1, 4 - 内酯脱氢酶, 脱氢抗坏血酸还原酶, 抗坏血酸过氧化物酶

Abstract: To investigate the relationship between ascorbate (AsA ) distribution and the activities of enzymes involved in AsA metabolism, the AsA and dehydroascorbate (DHA) contents and the activities of six related enzymes were studied in various organs of potato, i1e. young leaves, mature leaves, aged leaves, stems and tubers. The results showed that AsA was accumulated in young leaves and tubers. AsA pool size (the sum of AsA and DHA) in leaves and stemswas correlated with L-galactono-1, 4-lactone dehydrogenase (GalLDH) activity. AsA content was significantly related with the dehydroascorbate reductase (DHAR ) activity in all organs, especially in tubers. Meanwhile, DHA content was significantly associated with ascorbate peroxidase (APX) activity. This suggested that high AsA content in young leavesmight result from higher activites of GalLDH and DHAR, while AsA accumulation in tubermight be attributed to the transportation from leaves, and DHA recycling via DHAR.

Key words: potato, ascorbate, L-galactono-1, 4-lactone dehydrogenase, dehydroascorbate reductase, ascorbate peroxidase

中图分类号:

S 532

秦爱国;于贤昌. 马铃薯抗坏血酸含量及其代谢相关酶活性关系的研究[J]. 园艺学报, 2009, 36(9): 1370-1374.

QIN Ai-guo;YU Xian-chang. Relationship Between Ascorbic Acid Content and the Enzyme ActivitiesInvolved in AsA Metabolism of Potato[J]. ACTA HORTICULTURAE SINICA, 2009, 36(9): 1370-1374.

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