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园艺学报 ›› 2009, Vol. 36 ›› Issue (9): 1365-1369.

• 研究报告 • 上一篇    下一篇

四种寄主来源的灰葡萄孢对草莓的致病力分化研究初报

李喜玲;高智谋*; 李艳梅;邱艳;刘小燕;彭梅   

  1. (安徽农业大学植物保护学院, 合肥230036)
  • 收稿日期:2009-05-28 修回日期:2009-08-24 出版日期:2009-09-25 发布日期:2009-09-25
  • 通讯作者: 高智谋

Studies on Differentiation of Pathogenicity of Botrytis cinerea Isolates fromFour Hosts to Strawberry

LI Xi-ling, GAO Zhi-mou*;LI Yan-mei;QIU Yan;LIU Xiao-yan;PENGMei   

  1. (College of Plant Protection, Anhui Agricultural University, Hefei 230036, China)
  • Received:2009-05-28 Revised:2009-08-24 Online:2009-09-25 Published:2009-09-25
  • Contact: GAO Zhi-mou

摘要: 从合肥、蚌埠、长丰、和县等地采集的番茄、辣椒、草莓、葡萄等发病组织上分离鉴定获得23个灰葡萄孢菌株, 采用菌丝块创伤接种法, 分别测定了这些菌株对草莓果实和叶片的致力。结果初步表明, 所有供试菌株接种草莓果实和叶片后均引起发病, 但病斑的平均直径有显著差异, 并在不同草莓品种上表现也有差异, 显示灰葡萄孢菌株间对草莓果实和叶片的致病力存在明显的分化。按照在草莓果实和叶片所致病斑的平均直径大小可将供试菌株划分为致病力较强、中等和较弱3种类型。总体来说, 来自草莓和番茄的菌株对草莓果实和叶片的致病力较强, 来自辣椒的菌株对草莓果实和叶片的致病力较弱, 但来自相同寄主的菌株间致病力也存在差异, 菌株致病力差异与菌株地域来源无明显相关。

关键词: 灰葡萄孢, 致病力分化, 草莓, 寄主

Abstract: Twenty-three isolates of Botrytis cinerea Pers. were obtained from the diseased tissue collected in Hefei, Bengbu, Changfeng and Hexian, bymeans of tissue isolatingmethod. The pathogenicity of the isolates of B. cinerea from different hosts to the fruits and leaves of strawberrywere investigated by app lyingmycelial block wound inoculation, respectively. The preliminary results showed that all of the tested isolates caused occurrence of greymould on strawberry fruits and leaves, but there be significant difference in the average diameters of the lesions caused by different isolates and there was difference in the different strawberry varieties, suggesting that there was significant differentiation in pathogenicity of B. cinerea to strawberry fruits and leaves among isolates. According to the average diameters of the lesions caused to strawberry fruits and leaves, the pathogenicity of the all isolateswas classified into three categories: strong, intermediate and weak. In general, the isolates from strawberry and tomato were more strongly pathogenic to the fruits and leaves of strawberry, and the isolate from cap sicum was weakly pathogenic to strawberry fruits and leaves. However, there was difference in pathogenicity among the different isolates from the same host, and the pathogenicity difference was not obviously related to the localities of isolates

Key words: Botrytis cinerea, pathogenicity differentiation, strawberry, host

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