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园艺学报 ›› 2009, Vol. 36 ›› Issue (9): 1327-1332.

• 观赏植物 • 上一篇    下一篇

嫁接提高菊花耐高温与抗氧化能力的研究

房伟民1;郭维明1*; 陈俊愉2   

  1. (1南京农业大学园艺学院, 南京210095; 2北京林业大学园林学院, 北京100083)
  • 收稿日期:2009-04-13 修回日期:2009-08-19 出版日期:2009-09-25 发布日期:2009-09-25
  • 通讯作者: 郭维明

Effects of Grafting on the Improvement of Heat Tolerance and AntioxidantAbilities in Leaves of Chrysanthemum

FANGWei-min1;GUO Wei-ming1*;CHEN Jun-yu2   

  1. (1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2 College of Landscape A rchitecture, Beijing Forestry University, Beijing 100083, China)
  • Received:2009-04-13 Revised:2009-08-19 Online:2009-09-25 Published:2009-09-25
  • Contact: GUO Wei-ming

摘要: 比较了高温胁迫过程中以黄蒿和白蒿为砧木的菊花嫁接苗和扦插苗叶片膜透性、超氧阴离子自由基O2产生速率、超氧化物歧化酶( SOD) 和过氧化氢酶(CAT) 、抗坏血酸过氧化物酶(APX) 活性及可溶性蛋白质含量等的变化。结果表明:与扦插苗比较, 嫁接苗叶片膜透性和O2 产生速率较低, SOD、CAT与APX活性较高, 可溶性蛋白质的含量也相对较高, 而两种不同砧木的嫁接苗活性氧及抗氧酶活性等无显著差异。高温下嫁接苗叶片卷缩、萎蔫和黄化等症状均不同程度好于扦插苗。嫁接有利于菊花耐热能力的提高。

关键词: 菊花, 热胁迫, 嫁接, 抗氧化酶

Abstract: Using Artemisia frigida and Artemisia scoparia as root stocks, grafted chrysanthemum were created. The membrane permeability, O-·2 p roduction rate, and activities of superoxide dismutase ( SOD ) , peroxidase ( POD) , catalase (CAT) , ascrobate peroxidase (APX) and contents of soluble p rotein in leaves of the cutting seedlings and grafted seedlings of chrysanthemum were studied under heat stress. The results showed that grafted seedlings had lowermembrane permeability and O2production rate, while SOD, CAT and
APX activities and soluble p rotein contentswere higher compared with those in the cutting seedlings. However, there was no significant difference in O2production rate and activities of antioxidant enzymes between the grafted seedlingswith different root stocks. The leaf curling, wilting, yellowingwere alleviated in grafted seedling than those in cutting seedlings under heat stress. It suggested that grafting could imp rove the heat tolerance of chrysanthemum.

Key words: heattolerance, chrysanthemum, grafted, antioxidant enzymes activities

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