结球甘蓝相对于大白菜的连锁群特异SSR标记建立

园艺学报 ›› 2009, Vol. 36 ›› Issue (8): 1221-1226.

结球甘蓝相对于大白菜的连锁群特异SSR标记建立

顾爱侠;王彦华;轩淑欣;陈雪平;申书兴^*

(河北农业大学园艺学院, 河北保定071000)

收稿日期:2009-03-24 修回日期:2009-06-23 出版日期:2009-08-25 发布日期:2009-08-25
通讯作者: 申书兴

Establishment of Specific SSR from Different Linkage Groups of CabbageCompared with Chinese Cabbage

GU Ai-xia;WANG Yan-hua;XUAN Shu-xin;CHEN Xue- ing;SHEN Shu-xing^*

(College of Horticulture, Agricultural University of Hebei,Baoding,Hebei0 71001, China)

Received:2009-03-24 Revised:2009-06-23 Online:2009-08-25 Published:2009-08-25
Contact: SHEN Shu-xing

摘要/Abstract

摘要： 选用位于甘蓝9个连锁群的91对SSR引物, 对8个结球甘蓝品种和7个大白菜品种进行PCR
扩增, 筛选出25对结球甘蓝相对大白菜的特异SSR引物, 涉及甘蓝的9个连锁群, 其中位于O1连锁群3对, O2连锁群4对, O3连锁群2对, O4连锁群3对, O5连锁群2对, O6连锁群2对, O7连锁群4对,O8连锁群2对, O9连锁群3对。为利用SSR标记鉴定大白菜—结球甘蓝异附加系中附加的结球甘蓝染色体奠定了基础。

关键词: 大白菜, 结球甘蓝, SSR, 连锁群特异标记

Abstract: Ninety-one primers from 9 cabbage linkage group swere amp lified on 8 cabbage cultivars and 7 Chinese cabbage cultivars, 25 of them were specific on cabbage compared with Chinese cabbage. These primers assigned 9 linkage groups of cabbage, 3 of them were assigned linkage group O1, 4 of them were assigned linkage group O2, 2 of them were assigned linkage group O3, 3 of them were assigned linkage group O4, 2 of them were assigned linkage group O5, 2 of them were assigned linkage group O6, 4 of them were assigned linkage group O7, 2 of them were assigned linkage group O8, and 3 of them were assigned linkage group O9. It laid a foundation to further dentify the introduced chromosome of cabbage in Chinese cabbage alien addition lines.

Key words: Chinese cabbage, cabbage, SSR, linkage group-specific marker

中图分类号:

顾爱侠;王彦华;轩淑欣;陈雪平;申书兴. 结球甘蓝相对于大白菜的连锁群特异SSR标记建立[J]. 园艺学报, 2009, 36(8): 1221-1226.

GU Ai-xia;WANG Yan-hua;XUAN Shu-xin;CHEN Xue- ing;SHEN Shu-xing. Establishment of Specific SSR from Different Linkage Groups of CabbageCompared with Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2009, 36(8): 1221-1226.

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