Abstract: UV irradiated protoplasts ( donor protoplasts) isolated from petaloid cytoplasmic sterile line 7-0-8 were fused with 15 mM iodoacetamide treated protoplasts ( recipient protoplasts) isolated from fertile material 66-3 by using electronic fusion method, 33 plants were regenerated from fusion cells. All regenerated plants were diploid with chromosome number of 18, and they possessed the identical vegetative morphology to the recipient parent 66􀀁3.
In PCR amplification with mtDNA specific primers ( STS4 primer combination) , all regenerated plants obtained one same specific band which was identical to donor parent and different from recipient parent. The results indicated that
all regenerated plants were cybrids. In this experiment, 4 plants identified for their flower morphology were petaloid male sterile, which proved that intraspecific cybrids in carrot were obtained by asymmetric protoplast fusion and car rot petaloid cytoplasmic male sterility was successfully transferred from donor parent line 7-0-8 to fertile line 66-3.

Key words: Cybrids, Carrot ( Daucus carota L. ), Asymmetric protoplast fusion, Cytoplasmic male sterility