基于表型和SRAP 标记的切花菊品种遗传多样性分析

园艺学报 ›› 2014, Vol. 41 ›› Issue (1): 118-130.

基于表型和SRAP 标记的切花菊品种遗传多样性分析

张冬菊1,2，李世超1，吴鹏夫1，张晓1，李秋香1，杨树华1，贾瑞冬1，葛红1,*

1 中国农业科学院蔬菜花卉研究所，北京 100081；2 华中农业大学园艺林学学院，武汉 430070

出版日期:2014-01-25 发布日期:2014-01-25
基金资助:
国家‘863’计划课题（2011AA100208）；农业部‘948’计划项目（2012-G17）；公益性（农业）行业科研专项（200903008）；
中国农业科学院基本科研业务费项目（00320212016）；中国农业科学院科技创新工程项目；农业部园艺作物生物学与种质创制重点实验室
项目

Genetic Diversity Analysis in Cut Chrysanthemum Cultivars Based on Morphology and SRAP Markers

ZHANG Dong-ju1,2，LI Shi-chao1，WU Peng-fu1，ZHANG Xiao1，LI Qiu-xiang1，YANG Shu-hua1，JIA Rui-dong1，and GE Hong1,*

1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2College of
Horticulture & Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China

Online:2014-01-25 Published:2014-01-25

摘要/Abstract

摘要： 利用45 个表型性状和SRAP 标记分析56 个切花菊品种的遗传多样性。表型变异分析结果
表明：21 个性状表现出品种内一致性高及品种间特异性强；主成分分析发现，主成分贡献值较大的性状
有花序直径、花序类型和瓣型等花部性状，其次是叶部和茎杆性状，说明所选用的切花菊品种在分类时
应以花部性状为主，叶部和茎杆性状为辅；表型性状基于遗传距离UPGMA 聚类，将56 个切花菊品种分
为平瓣类、匙瓣类、桂瓣类、匙瓣—平瓣类和管瓣类，聚类结果大致按照花径—瓣型—花型分类。14 对
SRAP 引物组合扩增56 个切花菊品种的DNA，共扩增出454 条带，其中多态性带423 条，占扩增带数的
93.17%，多态性含量PIC 值在0.72 ~ 0.89 之间，平均为0.82，说明切花菊品种在分子水平上具有丰富的
遗传多样性。基于SRAP 标记的UPGMA 聚类分析显示：品种间遗传相似系数在0.64 ~ 0.97 之间，将56
个切花菊品种分为平瓣类—匙瓣、桂瓣类和管瓣类，聚类结果大致按照花径—瓣型—花型分类。Mantel
检验相关性系数为0.682，两种聚类结果有相似之处，均能很好体现试验切花菊品种间的遗传关系。

关键词: 切花菊, 表型性状, SRAP 标记, 品种分类, 遗传多样性

Abstract: Based on phenotypic analysis and SRAP markers，genetic diversity among 56 cultivars of
cut chrysanthemum were analyzed. Variation analysis showed that there were 21 characters which
presented the high consistency within cultivars and the distinct specificity between cultivars. Principal
component analysis showed that flower characters such as inflorescence diameter，inflorescence form and
petal type et al contributed mainly to the principal components，while leaf and stem characters as a secondary contribution. The results indicated that it would be necessary to primarily consider the flower traits，then
leaf and stem traits，as the criterion of classification and identification for cut chrysanthemum. Phenotypic
UPGMA clustering analysis showed that 56 cut chrysanthemum cultivars were classified into flat type，
spatulate type，irregular type，spatulate-flat type and tubiform type groups，which were mainly according
to three-grades classification as Inflorescence diameter–Petal type–Inflorescence form. 14 SRAP primer
pairs were screened and generated 423 polymorphic bands among 454 bands in total，which the percentage
of polymorphic bands reached 93.17%. The values of polymorphism information content（PIC）ranged
from 0.72 to 0.89 with an average of 0.82. These results indicated there was abundant genetic diversity
among cut chrysanthemum cultivars. UPGMA clustering analysis with SRAP markers showed 56 cultivars
with the genetic coefficient ranged from 0.64 to 0.97 were divided into spatulate-flat type，irregular type
and tubiform type groups，which were also according to three-grades classification as Inflorescence
diameter–Petal type–Inflorescence form. Mantel’s test with correlation coefficient of 0.682 indicated that
the high accordance between phenotypic and molecular markers analysis，suggesting both analysis could
well reflect the genetic relationship of cut chrysanthemum cultivars.

Key words: cut chrysanthemum, phenotypic characters, SRAP marker, cultivar classification, genetic
diversity

中图分类号:

S 682.1⁺1

张冬菊, 李世超, 吴鹏夫, 张晓, 李秋香, 杨树华, 贾瑞冬, 葛红. 基于表型和SRAP 标记的切花菊品种遗传多样性分析[J]. 园艺学报, 2014, 41(1): 118-130.

ZHANG Dong-Ju, LI Shi-Chao, WU Peng-Fu, ZHANG Xiao, LI Qiu-Xiang, YANG Shu-Hua, JIA Rui-Dong, GE Hong. Genetic Diversity Analysis in Cut Chrysanthemum Cultivars Based on Morphology and SRAP Markers[J]. ACTA HORTICULTURAE SINICA, 2014, 41(1): 118-130.

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