关键词: 柿, 丙烯, 1–甲基环丙烯, XTH, 采后, 实时荧光定量PCR

Abstract: In order to further explore the molecular regulation mechanism of xyloglucan endotransglycosylase/ hydrolase（XTH）during‘Fuping Jianshi’persimmon（Diospyros kaki L.）postharvest softening，the responses of XTH genes in persimmon fruit to propylene and 1-MCP at the room temperature were investigated by real-time quantitative PCR（RT-qPCR）approach. The results showed that treatment with propylene promoted the softening of persimmon fruits，increased the ethylene production and advanced the onset of climactic of ethylene production；While treatment with 1-MCP delayed the
softening of persimmon fruits，and inhibited ethylene production. Accompanying with fruit ripening，four XTHs genes showed different expression patterns. Propylene treatment promoted the expression of four XTHs genes while 1-MCP treatment inhibited the expression of four genes. The expression levels of
DkXTH1 and DkXTH2 were high during persimmon fruit softening，and the expression peaks of two genes were appeared in the early stage and middle stage，respectively. These results indicated that fruit softening was related to both DkXTH1 and DkXTH2 expression in‘Fuping Jianshi’persimmons and DkXTH1 might involve in the early stage while DkXTH2 was associated with the later stage of fruit softening. Therefore， the expression of XTH genes in persimmon fruits was concluded to be regulated by ethylene and was related to persimmon fruit softening.

Key words: persimmon, propylene, 1-MCP, XTH, postharvest, real-time quantitative PCR