关键词: 柑橘, 异源四倍体体细胞杂种, SSR 标记, 遗传分析, 三倍体后代, 双二倍体

Abstract: Two pairs of SSR（simple sequence repeat）primers TAA1 and TAA3 were used to detect the band patterns and segregation rations among the 79 triploid progenies derived from the sexual cross between diploid Citrus grandis（L.）Osbeck‘Shatian’pummelo and somatic hybrid NS [（C. reticulata
Blanco × C. paradisi Macf.）‘Nova’tangelo + C. sinensis（L.）Osbeck‘Succari’sweet orange] by embyo rescue technology. Five and four band patterns were generated from the TAA1 and TAA3 primers at the ratio of 4︰1︰1︰5︰1 and 2︰2︰1︰1 respectively，as was in good accordance with the segregation ratio of amphidiploid deduced by the law of Mendel. The results preliminarily indicated that the meiosis behavior of citrus allotetraploid somatic hybrid was similar to that of the amphidiploid.

Key words: citrus, allotetraploid somatic hybrid, SSR marker, genetic analysis, triploid progeny, amphidiploid